High-resolution MRI characterization of human thrombus using a novel fibrin-targeted paramagnetic nanoparticle contrast agent.
نویسندگان
چکیده
In this study, the sensitivity of a novel fibrin-targeted contrast agent for fibrin detection was defined in vitro on human thrombus. The contrast agent was a lipid-encapsulated perfluorocarbon nanoparticle with numerous Gd-DTPA complexes incorporated into the outer surface. After binding to fibrin clots, scanning electron microscopy of treated clots revealed dense accumulation of nanoparticles on the clot surfaces. Fibrin clots with sizes ranging from 0.5-7.0 mm were imaged at 4.7 T with or without treatment with the targeted contrast agent. Regardless of sizes, untreated clots were not detectable by T(1)-weighted MRI, while targeted contrast agent dramatically improved the detectability of all clots. Decreases in T(1) and T(2) relaxation times (20-40%) were measured relative to the surrounding media and the control clots. These results suggest the potential for sensitive and specific detection of microthrombi that form on the intimal surfaces of unstable atherosclerotic plaque.
منابع مشابه
Novel MRI contrast agent for molecular imaging of fibrin: implications for detecting vulnerable plaques.
BACKGROUND Molecular imaging of thrombus within fissures of vulnerable atherosclerotic plaques requires sensitive detection of a robust thrombus-specific contrast agent. In this study, we report the development and characterization of a novel ligand-targeted paramagnetic molecular imaging agent with high avidity for fibrin and the potential to sensitively detect active vulnerable plaques. MET...
متن کاملGlucosamine Conjugated Gadolinium (III) Oxide Nanoparticles as a Novel Targeted Contrast Agent for Cancer Diagnosis in MRI
Background: Glucose transporter (Glut), a cellular transmembrane receptor, has a key role in the metabolism of cell glucose and is also associated with various human carcinomas.Objective: In this study, we evaluated a magnetic resonance (MR) imaging contrast agent for tumor detection based on paramagnetic gadolinium oxide (Gd2O3) coated polycyclodextrin (PCD) and mod...
متن کاملChronic thrombus detection with in vivo magnetic resonance imaging and a fibrin-targeted contrast agent.
BACKGROUND Arterial thrombosis plays a critical role in acute coronary syndromes and stroke. Therefore, the ability to detect thrombus in vivo has a significant clinical implication. Magnetic resonance imaging (MRI) has shown promise in noninvasive thrombus detection. However, thrombus characterization and age definition remain difficult. We sought to evaluate the use of a fibrin-targeted pepti...
متن کاملMulti-Modality PET-MR Perfluorocarbon Nanoparticle Contrast Agent for Ligand-Targeted Quantitative Imaging
Introduction. The combination of positron emission tomography and magnetic resonance imaging offers a major breakthrough in detecting, characterizing and monitoring disease. This work introduces a novel contrast agent, detected by both modalities, which employs a self-inserting chelator with linker peptide derived from melittin (a host defense peptide in honeybee venom). Based on a lipid-encaps...
متن کاملRecent Advances in PET-MR Hybrid contrast agent
Introduction: All of the Imaging modalities have advantages and disadvantages alone. So if we want to have the best and perfect image, combining these modalities produces something we desired. PET-MR images consist of morphologic and metabolic data. MRI and PET provide high spatial and contrast resolution and high sensitivity and molecular information respectively. Hybrid PET-...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Magnetic resonance in medicine
دوره 44 6 شماره
صفحات -
تاریخ انتشار 2000